BBI-AGTC

The Chromium Controller has been obsolesced by 10x Genomics. It will be kept in operation at AGTC as long as it remains functional.

All assays include partitioning and barcoding using the Chromium Controller and manual library preparation.

Chromium Single Cell Multiome ATAC + Gene Expression

Simultaneous gene expression and open chromatin profiling from the same cell for tens of thousands of cells
- Link regulatory elements and target genes
- Characterize cell heterogeneity
- Discover new gene regulatory interactions
- Identify rare cell types
Input:
- 500 - 10,000 nuclei
Suitable for:
- Nuclei treated with transposase
Molecular barcoding and capture:
- Capture and amplify transposase-accessible DNA fragments
- Capture and amplify 3' mRNA

Assay for transposase-accessible chromatin (ATAC) for epigenomic analysis of tens of thousands of individual nuclei
- Define cell types and states
- Catalog cell type–specific regulatory elements
- Identify important transcription factors
- Characterize gene regulatory networks
Input:
- 500 - 10,000 nuclei
Suitable for:
- Nuclei treated with transposase
Molecular barcoding and capture:
- Capture and amplify transposase-accessible DNA fragments

10x Genomics is ending the sale of this kit at the end of 2025 and it will no longer be offered.

Gene expression profiling for characterization of tens of thousands of single cells
- Identify rare cell types
- Atlas and characterize complex cell populations
- Understand cellular heterogeneity
- Discover new biomarkers
Input:
- 500 - 10,000 cells or nuclei
Suitable for:
- Cells or nuclei
- Flow-sorted cells
- Cells labeled with cell surface protein antibodies
- Cells expressing compatible CRISPR guides
Molecular barcoding and capture:
- Capture and amplify 3' mRNA
- Capture and identify cell surface proteins and CRISPR perturbations

10x Genomics is ending the sale of this kit at the end of 2025 and it will no longer be offered.

Paired, full-length receptor sequencing and gene expression profiling for tens of thousands of T and B cells
- Profile immune cell repertoires
- Determine antigen specificity of B cells and T cells
- Characterize tissue microenvironments
- Go beyond traditional cytometry
Input:
- 500 - 10,000 cells
Suitable for:
- Cells
- Flow-sorted cells
- Cells labeled with cell surface protein antibodies
- Cells labeled for antigen specificity analysis
Molecular barcoding and capture:
- Capture and amplify 5' mRNA
- Capture and sequence paired, full-length BCR/TCR genes
- Capture and identify cell surface proteins and antigen-specific immune receptors

Requests for projects requiring significant modifications to existing protocols or library preparation options not already listed here will be discussed with the customer on a per-project basis.
AGTC is piloting the Illumina Single Cell 3' RNA Prep as an alternative to the 10x 3' Gene Expression kit. Contact us for more information.

We cannot accept any samples that would be classified as Biosafety Level 2 (BSL2) or higher!
We work closely with the customer to coordinate sample submission and workflow timelines to optimize cell viability and recovery.
The maximum cell size 10x Genomics tested in-house is 30 μm. 10x Genomics did not observe differential recovery for small vs. large cells when running a sample composed of cells of different sizes (for cells that are equal to or smaller than 30 μm).
The recommended starting point for a new sample type is to load ~1,700 cells into each reaction, recovering approximately 1,000 cells, to achieve an expected multiplet rate of approximately 0.8%. For comparison, loading ~17,400 cells and recovering approximately 10,000 cells yields a multiplet rate of approximately 7.6%.