A NGS library is a collection of similarly sized DNA fragments from a single sample which have known indexes and adapter sequences added to the 5' and 3' ends of each fragment. A barcode or index is a unique 6-10 bp identifier used to distinguish fragments of one library from another. These index and adapter sequences facilitate the process of sequencing by synthesis.
The latest library preparation kits from Illumina, NEB, and 10x Genomics allow for:
- Lower input requirements
- Higher library yield
- Better percent detection
- Lower duplicate rate
- Better alignment
- Accurate base calling and variant identification
- Greater assay sensitivity
- Compatibility with degraded and FFPE samples
Library prepreparation kits used include:
- DNA - Illumina DNA PCR-Free Prep, Illumina DNA Prep, NEBNext Ultra II [FS] DNA Prep
- RNA - Illumina Stranded Total RNA Prep with Ribo-Zero Plus, Illumina Stranded mRNA Prep
- Single Cell - 10x Genomics Single-Cell/Nucleus Gene Expression