Library Preps - DNA

Illumina DNA PCR-Free Prep, Tagmentation

  • Whole Genome Sequencing
    • Delivers consistent insert sizes, uniform coverage, and optimized performance
    • Bead-based technology minimizes bias and opportunities for error
    • PCR-free workflow avoids PCR duplicates
    • On-bead fragmentation
    • No library quantification needed
    • Very quick turn-around time
    • How it works
  • Suitable for:
    • Human DNA WGS
    • Multiple sample types
    • Small and large genomes
    • Recommended replacement for the TruSeq DNA PCR-Free kit
  • Required DNA input (in a volume ≤ 25 μL):
    • 25 - 2000 ng
  • Includes:
    • 10 bp unique dual indexes (Illumina DNA/RNA UD Indexes, Tagmentation)
    • Multiplexing of up to 384 samples

Illumina DNA Prep, (M) Tagmentation

  • Previously called the Nextera DNA Flex kit
  • Whole Genome Sequencing
    • Delivers consistent insert sizes (~350 bp), uniform coverage, and optimized performance
    • Bead-based technology minimizes bias and opportunities for error
    • PCR-based workflow
    • On-bead fragmentation
    • No library quantification needed
    • Quick turn-around time
  • Suitable for:
    • DNA WGS with low input
    • Multiple sample types
    • Flexibility to sequence human or other large, complex genomes as well as amplicons or microbial species
    • Recommended replacement for the Nextera DNA Library Prep kit
  • Required DNA input (in a volume ≤ 30 μL):
    • 1 - 500 ng
  • Includes:
    • 10 bp unique dual indexes (IDT for Illumina Nextera DNA UD Indexes)
    • Multiplexing of up to 384 samples (using unique dual indexes)

NEBNext Ultra II DNA / NEBNext Ultra II FS DNA

  • Higher library yields from lower input amounts
    • Generates high yields with increased reaction efficiencies and minimized sample loss
    • Reliable fragmentation with a single protocol, regardless of DNA input amount or GC content (optional)
    • PCR-based workflow
    • Tunable bead-based size selection to select for insert sizes ranging from 150 to 700 bp
    • Quick turn-around time
  • Suitable for:
    • DNA with ultra-low input
    • Multiple sample types including GC-rich and FFPE
    • Genomic DNA, fragmented DNA, or amplicons
  • Required DNA input:
    • 0.5 - 1000 ng (in a volume ≤ 50 μL, without fragmentation)
    • 0.1 - 500 ng (in a volume ≤ 26 μL, with fragmentation)
  • Includes:
    • Fragmentation (optional), end repair, dA-tailing, adapter ligation, PCR and index incorporation
    • 8 bp unique dual indexes (NEBNext Multiplex Oligos for Illumina - UDI Primer Pairs)
    • Multiplexing of up to 384 samples (using unique dual indexes)

Other Library Preparation Options

  • Requests for projects requiring significant modifications to existing protocols or library preparation options not already listed here will be discussed with the customer on a per-project basis.