Grant Writing

Facilities and Equipment - The Advanced Genomic Technologies Core of the Brain & Behavior Institute (BBI-AGTC) at University of Maryland

The Advanced Genomic Technologies Core of the Brain & Behavior Institute (BBI-AGTC) is a full-service sequencing facility serving the University of Maryland community and external clients. Situated in the Bioscience Research Building at the heart of the life science community on campus, the mission of the BBI-AGTC is to make state-of-the-art genomic technologies accessible to researchers at a reasonable cost. Services and technologies offered include DNA and RNA qualitative and quantitative analysis, high-throughput sequencing (up to 800M reads per run), whole genome library preparation and sequencing, RNA library preparation - including stranded RNA prep with rRNA depletion, and single-cell genomic/expression profiling. Equipment includes a sequencer (Illumina NextSeq 1000), a single cell controller (10X Genomics Chromium), an automated liquid handling station (Eppendorf epMotion 5075tc), a real-time PCR machine (Roche LightCycler 96), an Agilent TapeStation 4150, and a high performance ultrasonicator for DNA shearing (Covaris SureSelect S2).

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Acknowledging the BBI-AGTC

The existence of core facilities depends in part on proper acknowledgement in publications. Please acknowledge the help of the Brain & Behavior Institute - Advanced Genomic Technologies Core (BBI-AGTC) in your reports and publications.

Evidence of our contribution to your scientific output is a key metric for the management of the core, as it allows us to 1) assess our scientific impact, 2) justify institutional investment, and 3) eventually pursue external grant funding with the ultimate goal of ensuring a continuous operation and providing our essential services to the scientific community.

Please use the following text in all publications:

"Part of this work was conducted at the Brain & Behavior Institute - Advanced Genomic Technologies Core (BBI-AGTC), which is supported by the BBI and the University of Maryland, College Park."

It would be also appreciated if you sent a copy of the accepted manuscript or published paper to bbi-agtcumd.edu. This would facilitate our task when we prepare our yearly institutional report.

Enrichment Workshops

January 26, 2022

This workshop was held on January 26, 2022, 12:00-1:00 pm on Zoom. The presentations are posted below and accessible to those affiliated with UMCP. Unfortunately, we ran out of time and did not want to rush through the second presentation which will be presented in full at a later date.

  • DNA/RNA quality and quantity evaluation for NGS workflows – April Hussey (Core Manager)
    • Presentation slides with additional notes (portrait): PDF
    • Presentation slides only (landscape): PDF
    • Recording: MP4
  • Considerations for DNA-seq and RNA-seq read length and coverage – Najib El-Sayed (Core Director)
May 5, 2021

This workshop was held on May 5, 2021 on Zoom. The presentation is posted below and accessible to those affiliated with UMCP.

  • Biology at True Resolution: Resolving Biology with single-cell genomics, multi-omics, and spatial transcriptomics – Bradley Toms, M.S. (10X Science & Technology Advisor)
    • Presentation slides (landscape): PDF
    • Recording: MP4
April 21, 2021

This workshop was held on April 21, 2021 on Zoom. The presentation is posted below and accessible to those affiliated with UMCP.

  • NextSeq1000, Enabled Aapplications, and Informatics @ BBI-AGTC – Dan Gheba, Tara Kesteloot, Bao Ho (Illumina Team)
    • Presentation slides (landscape): PDF
    • Recording: MP4